Focusrite ISA One To Scarlett 2i2 (3rd Gen)

[KXNG iLLuSioN]

Hello everyone,

New here and I was wondering if I may get some advice.

Gear:
Microphone: TLM 103
Interface: Scarlett Focusrite 2i2 (3rd gen)
Pre-Amp: Focusrite ISA One
Headphones: DT beyerdynamic DT 900 PRO 250ohm
Acoustic Treatment
Portable Vocal Shield

DAW:
Pro Tools 12

OPS: Windows 11


I only record vocals and its usually to (Leased WAV) 2 track hip hop beats.
I have the preamp and interface wired correctly and I can record into pro tools with no issues. I'm trying to get professional sounding vocals. I have been messing around with "gain staging", I have watched countless you tube videos and was wondering if there is any extra advice out there for me.

As in Should I be driving the Input or Trim knob more on the ISA One?

My TLM 103 has a level of 50 ohms so should my impedance on the ISA One be low (600 ohms) or the ISA 110 (1400 ohms) or even higher?

Should I be recording my sessions in 44.1hz, 48hz or ect?

Sorry if its confusing and thank you in advanced