No signal on input

[BenMeyers]

I'm on a quest to use Pro Tools to run our church live stream. We have a Presonus 32R mixer for the broadcast. I had been having a problem with the mixer not being available on the computer, but that decided to just remedy itself after a few weeks. On Sunday I did a test recording to make sure it would work. I created 32 tracks and armed everything. I recorded every channel. When went to go mix it, I didn't have any drum tracks. Today I went to church and tried recording some drum samples myself so I could get the signal processing set up before Sunday morning. made sure I had the correct inputs but had no signal. I reset the I0. No change. There's signal on the mixer and I'm running Windows. Anyone have any ideas?