First thing to do is look near the top of this(and every) DUC page for the link named
Help Us Help You and have a good read. Its nearly impossible to offer any help with almost zero info on the system("PC" and "PT-Studio" constitutes maybe 1% of the required info). Post a Sandra Lite report and include details on the interface and exact build versions of PT and OS.
Steps worth trying:
1-trash prefs(guitardom's Trasher utility available at pro-tools-pc.com is the best option for Windows machines).
2-If your system drive is a fast SSD, try running your session on that drive(a separate recording drive is ideal, but with the advent of fast SSD drives, its not the "requirement" that it once was).
3-you mention a "small glitch". So what might be causing this glitch? Turn off all networking and see if that helps. Open Task Manager and see what programs are running in the background. Have you performed ALL the required system tweaks?
4 and up will be a mystery until you provide complete system details
