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Hello Eddie, thanks for your reply!
I did what you asked me, but it made no difference. EuControl still could not make a connection between my workstation and the application "TotalMixFX"

But then i tried something:
- I kiled TotalMix and the RME driver in system tray.
- deleted the Midi assignment in Eucon Contol Panel
- made a new assignment for MidiPort 1-4 to TotalMixFX (Mackie Protocol) in the Eucon Contol Panel
- resartet RME TotalMixFX

And then everything worked fine. But after a restart again no connection between workstation and totalmix...
so it seems to be a small problem somewhere in the midi assignment.

how can I fix this in a way that it also works after a restart?

Thanks in advance,
Martin